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licorbio intercept antibody diluent  (LI-COR)


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    Structured Review

    LI-COR licorbio intercept antibody diluent
    Licorbio Intercept Antibody Diluent, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intercept+antibody+diluent+buffer/Intercept+(TBS)+Blocking+Buffer+and+Diluent+Kit/pmc13080315-158-7-7
    Average 98 stars, based on 37 article reviews
    licorbio intercept antibody diluent - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Lung infection by Pseudomonas aeruginosa induces neuroinflammation and blood–brain barrier dysfunction in mice
    Article Snippet: Brain homogenates were run on a 4–20% Tris–Glycine gel (Mini-protean TGX gel; Bio-Rad laboratories; #4561094) and transferred onto a nitrocellulose membrane (TransBlot ® Turbo RTA mini nitrocellulose transfer kit; Bio-Rad laboratories; #1704270). .. The membranes were blocked in intercept blocking buffer (Li-Cor; #927-70001) for 1 h at room temperature, probed with primary antibodies against claudin-5 (1:250; Cell Signaling technology; #49564), occludin (1:500; BD Biosciences; #611091), VE-cadherin (1:1000; Abcam; ab205336), GFAP (1:1000; Sigma-Millipore; MAB360), and beta-actin (1:1000; Li-Cor; #926-42212 and #926-42210) diluted in intercept antibody diluent buffer (Li-Cor; #927-75001). .. Membranes were washed with PBS-T (0.1% Tween-20) and probed with Li-Cor IRDye secondary antibodies diluted in intercept antibody diluent buffer for 1 h at room temperature.

    Article Title: Lung infection by Pseudomonas aeruginosa induces neuroinflammation and blood-brain barrier dysfunction in mice.
    Article Snippet: Brain homogenates were run on a 4–20% Tris–Glycine gel (Mini-protean TGX gel; Bio-Rad laboratories; #4561094) and transferred onto a nitrocellulose membrane (TransBlot® Turbo RTA mini nitrocellulose transfer kit; Bio-Rad laboratories; #1704270). .. The membranes were blocked in intercept blocking buffer (Li-Cor; #927- 70001) for 1 h at room temperature, probed with primary antibodies against claudin-5 (1:250; Cell Signaling technology; #49564), occludin (1:500; BD Biosciences; #611091), VE-cadherin (1:1000; Abcam; ab205336), GFAP (1:1000; Sigma-Millipore; MAB360), and betaactin (1:1000; Li-Cor; #926-42212 and #926-42210) diluted in intercept antibody diluent buffer (Li-Cor; #927-75001). .. Membranes were washed with PBS-T (0.1% Tween-20) and probed with Li-Cor IRDye secondary antibodies diluted in intercept antibody diluent buffer for 1 h at room temperature.

    Membrane:

    Article Title: Epsin1 enforces a condensation-dependent checkpoint for ubiquitylated cargo during clathrin-mediated endocytosis
    Article Snippet: Proteins were transferred to a nitrocellulose membrane (BioRad), blocked for 1 hour at room temperature with LI-COR Block Buffer, and probed with an anti-Epsin1 antibody (mouse monoclonal, Santa Cruz, Product no. sc-55556)) diluted 1:1000 in 1x TNET Wash Buffer (10 mM Tris, pH 7.5, 2.5 mM EDTA, pH 8.0, 50 mM NaCl, 0.1% Tween-20). .. After three washes in TNET Wash Buffer (5 min each), the membrane was incubated for 1 hour at room temperature with secondary Goat anti-Mouse IgG (H + L) Alexa FluorTM Plus 680 antibody (Invitrogen, Product # A32729) diluted 1:15,000 in Intercept Antibody Diluent Buffer (LI-COR). .. Following another three washes in TNET Wash Buffer, the blot was imaged using a LI-COR Odyssey Imaging System.

    Incubation:

    Article Title: Epsin1 enforces a condensation-dependent checkpoint for ubiquitylated cargo during clathrin-mediated endocytosis
    Article Snippet: Proteins were transferred to a nitrocellulose membrane (BioRad), blocked for 1 hour at room temperature with LI-COR Block Buffer, and probed with an anti-Epsin1 antibody (mouse monoclonal, Santa Cruz, Product no. sc-55556)) diluted 1:1000 in 1x TNET Wash Buffer (10 mM Tris, pH 7.5, 2.5 mM EDTA, pH 8.0, 50 mM NaCl, 0.1% Tween-20). .. After three washes in TNET Wash Buffer (5 min each), the membrane was incubated for 1 hour at room temperature with secondary Goat anti-Mouse IgG (H + L) Alexa FluorTM Plus 680 antibody (Invitrogen, Product # A32729) diluted 1:15,000 in Intercept Antibody Diluent Buffer (LI-COR). .. Following another three washes in TNET Wash Buffer, the blot was imaged using a LI-COR Odyssey Imaging System.



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